Fig. 1. AR promotes VEGF-C expression and subsequently increases lymphangiogenesis in human chondrosarcoma cells. Cells were incubated with AR (3-50 μg/ml) for 24 h and then AR (50 μg/ml) for 6, 12 and 24 h; VEGF-C expression was examined by qPCR (A and B) and ELISA (C and D). (E and F) JJ012 cells were incubated with AR (3-50 μg/ml) for 24 h. The medium was collected as conditioned medium (CM) and JJ012 cells were pretreated with IgG/VEGF-C antibody (1 mg/ml) for 30 min, then incubated with AR for 24 h before being applied to LECs. LEC migration and capillary-like structure formation was examined by Transwell and tube formation assay (E and F). Results are expressed as the mean ± SEM. *P<0.05 compared with controls; #P<0.05 as compared with the AR-treated group.