Fig. 2. The Src pathway is involved in AR-induced VEGF-C expression and lymphangiogenesis in human chondrosarcoma cells. Cells were pretreated with PP2 for 30 min or transfected with siRNAs for 24 h, then stimulated with AR (50 μg/ml) for 24 h. VEGF-C expression was examined by qPCR (A) and ELISA (B). The medium was collected as CM and then applied to the LECs. Capillary-like structure formation and LEC migration was examined by tube formation and the Transwell assay, respectively (C and D). (E) JJ012 cells were incubated with AR (50 μg/ml) for 120 min, and Src phosphorylation was determined by Western blotting. Western blot data were normalized to Src as the loading control. Results are expressed as the mean SEM. *P<0.05 compared with controls; #P<0.05 compared with the AR-treated group.