Fig. 3. NF-kB signaling is involved in gliclazide-induced inhibition of VSMCs proliferation and migration. Western blot was used to determine the expression of p-NF-kB (A). CCK8 was used to evaluate proliferation of VSMCs (B). Wound healing assay and transwell assay were used to evaluate the migration of VSMCs (C, D, E). Data are expressed as the mean ± SEM of triplicate determinations. * P<0.05 versus control; # P<0.05 versus gliclazide. PMA is an NF-κB activator.