Fig. 3. Representative photographs of renal tissues sections stained with hematoxylin and eosin (H&E) from control rats (A), and rats treated with adenine (B), canagliflozin 10 mg/kg (C), canagliflozin 10 mg/kg + adenine (D), canagliflozin 25 mg/kg (E), and canagliflozin 25 mg/kg + adenine (F). No histological changes were seen in the control or the canagliflozin treated two groups. The adenine-treated group showed large areas of acute tubular injury, tubular distention with necrotic material involving tubules, loss of brush border of proximal tubules, dilatation of large number of tubules, mixed inflammatory cells infiltration of the interstitium and focal tubular atrophy. The two groups that received canagliflozin + adenine showed improvement in the histological appearance, with less dilated tubules, interstitial inflammation and atrophic tubules apoptotic cells.