Fig. 3. Regulation of ischemia reperfusion injury (IRI)-induced inflammation by substance P (SP) injection. (A) IRI-induced systemic inflammatory responses were evaluated by complete blood cell counting (CBC) analysis and (B-F) Enzyme-linked immunosorbent assays (ELISA) against TNF-α (B, C) and IL-10 (D) in the serum, and (E) fluorescence activated cell sorting (FACS) analysis of CD11b+ CD206+ M2-phenotype monocytes and (F) CD4+ Foxp3+ Treg cells in peripheral blood mononuclear cells (PBMCs). The data are shown as the mean SD. *p<0.05, **p<0.01, ***p<0.001.