Fig. 6. FOXO1 is involved in the regulatory effects of miR-486-5p in NP cells. (A) qRT-PCR was performed for the mRNA expressions of inflammatory cytokines. (B,C) qRT-PCR was performed for the mRNA expressions of ECM genes and matrix degrading enzymes. (D) Western blot was used to detect the expressions of apoptosis-related proteins. Bands were measured by densitometry with Quantity One quantitation analysis software package. Each experiment was assayed in triplicate independently. Data was represented as means SD. *P<0.001, v.s. control.