Fig. 4. MyD88 deficiency reduced the palmitate induced expression of MIP-1α. MyD88 KO human monocytic cells were incubated with 0.1% BSA (Vehicle) or palmitate (100Ám) or TNF-α (10ng/ml; MyD88 independent stimulus) or LPS (10ng/ml; TLR4/MyD88 dependent stimulus). Cells and culture media were collected after 24hrs. MIP-1α gene expression was determined by real time PCR (A) and secreted MIP-1α protein was determined in culture media by ELISA (B). The data are presented as mean ▒SEM. Statistical analysis was done using t-test. P<0.05 was considered as statistically significant. **P< 0.001.