Fig. 5. FIR irradiation increases the activity of Ca2+-mediated PP2B. (A) TMSC were acutely exposed to FIR irradiation for 30 min in media containing 1 μM Fluo-4 AM. Images of intracellular Ca2+ were visualized using a confocal microscope. Scale bar = 50 μm. (B) TMSC were exposed to FIR irradiation for 30 min. PP2B protein was immunoprecipitated using an anti-PP2B antibody and PP2B activity in the precipitates was measured using a PP2B cellular activity kit. Each bar represents the mean S.D. (n=3). Difference was statistically significant at *p<0.05.