Fig. 6. Effects of activation of Ca2+-mediated PP2B on adipogenic or osteogenic differentiation of TMSC. (A-B) After pretreatment with CsA (0, 0.25, 0.5, and 1 μM), TMSC were exposed to FIR irradiation for 30 min and further incubated in adipogenic differentiation medium for up to 14 days. Control cells without CsA pretreatment were used as Sham. (A) Accumulation of lipid droplets and (B) protein levels of PPARγ, FABP4, and GAPDH were measured as described in the legend of Fig. 2. (C-D) TMSC were pretreated with CsA before exposure to FIR irradiation, and further incubated in differentiation medium as described in the legend of Fig. 6A with the exception of using osteogenic medium instead of adipogenic medium. Control cells without CsA pretreatment were used as Sham. (C) Mineralization and (D) protein levels of osteocalcin, ALP, and GAPDH were measured as described in the legend of Fig. 2. The results are representative of three independent experiments, and each bar represents the mean S.D. Differences were statistically significant at *p<0.05, **p<0.01, and ***p<0.001.