Fig. 3. Wip1 is required for hypoxia-induced p53-Mdm2 interaction and apoptosis in HTR8/SVneo cells. A-C. Apoptosis of HTR8/SVneo cells after 24 h of HII (A) or SIB (B) in the presence of GSK (20 ÁM), DMSO (0.1%), shWip1, or scrambled shRNA (MOI=50), were determined by FCM, and apoptosis rate was qualified by percentage of Q2 and Q3 quadrants (C). n=4, *P<0.05, **P<0.01, one-way ANOVA, Sidak's multiple comparisons test; D. Co-IP of Mdm2 and p53 in HTR8/SVneo cells after 24 h of HII or SIB treatment in the presence of GSK (20 ÁM) or shWip1 (MOI=50). Experiments were performed in triplicate.