Fig. 7. Wip1 acts as a downstream effector of p53 in trophoblasts. Western blotting of p38-Wip1 loop consisting molecules in HTR8/SVneo cells pretreated with 2 ÁM NVP for 6 h, followed by 24 h of normoxia, HII, or SIB treatment in fresh medium along with 20 ÁM GSK or shWip1 (MOI=50), Kruskal-Wallis test, Dunn's multiple comparison test for p-p53/p53 in HII and one-way ANOVA, Dunnett-t multiple comparison test for others. n=4, β-actin was used as loading control. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Experiments were performed in triplicate.