Fig. 7. SOX9 is a target of miR-101-3p. (A) A schematic illustration of wt and Mut 3'-UTR of SOX9. (B) Dual-luciferase reporter assay revealed that miR-101-3p inhibited wt SOX9 3'-UTR luciferase activity, but not Mut SOX9 3'-UTR luciferase activity. (C) SOX9 expression was determined with western blot in A549 and SPCA1 cells transfected with miR-101-3p mimics or miR-NC. *, P<0.05.