Fig. 2. IL-1b and MCP-1 secreted by TNF-α activated monocytes are suppressed by ACSL1 inhibition. (A and B) Monocytes were pretreated with ACSL1 inhibitor (triacsin C: (1uM) or vehicle for 1 hour and then incubated with TNF-α for 24 hours. Secreted IL-1b and MCP-1 protein in culture media was determined by ELISA. All data are expressed as mean SEM (n ≥ 3). *p<0.05, **p<0.01, ***p<0.001 versus vehicle.