Fig. 3. Akt is involved in resistin-induced MCP-1 expression and monocyte migration. (A&B) OASFs were pretreated for 30 min with an Akt inhibitor (10 μM) or transfected with the Akt siRNA then stimulated with resistin. MCP-1 expression was examined by qPCR. (C&D) CM was applied to THP-1 cells and analyzed for migration activity. (E&F) OASFs were incubated with resistin or pretreated with PI3K inhibitors then stimulated with resistin and p-Akt expression was examined by Western blot. Results are expressed as the mean SEM. *p<0.05 as compared with the control group; #p<0.05 as compared with the resistin-treated group.