Fig. 2. miR-186-5p suppresses cell proliferation. (A) U2OS, MG-63 and Saos-2 cells were transiently transfected with miR-186-5p mimics or miR-NC, respectively. (B) CCK-8 assay was performed to detect the cell proliferation ability. (C) U2OS, MG-63 and Saos-2 cells expressing miR-186-5p or miR-NC were subcutaneously injected into the flanks of nude mice. The mice were closely monitored for tumor growth, and tumor size was measured at the indicated times. Photographs of tumors isolated from mice by surgical excision at the final experiment day. Values presented as mean ± SD. *P<0.001, v.s. control.