Fig. 3. miR-186-5p suppresses cell cycle and EMT, and induces apoptosis. (A) Cell cycle of OS cells was analyzed by flow cytometry. (B) Annexin/PI assay was used to detect apoptosis of OS cells. (C) Western blot was used to analyze EMT markers E-cadherin, N-cadherin, and Vimentin in OS cells transfected with miR-186-5p mimics or miR-NC. GAPDH served as an internal control. Values presented as mean ± SD. *P<0.001, v.s. control.