Fig. 6. FOXK1 is critical for the biological functions of miR-186-5p in OS cells. (A) Expression of FOXK1 in U2OS, MG-63 and Saos-2 cells after co-transfection of miR-186-5p mimics with si-control or si-FOXK1. And then CCK-8 assay was performed to detect the cell proliferation ability. (B,C) Transwell assay was performed to determine the migration and invasion ability. Values presented as mean ± SD. *P<0.001, v.s. control.