Fig. 5. Macrophages are the predominant source of IL-10 production in AT-treated septic mice. A) IL-10 levels were quantified from blood 90 minutes after LPS challenge; *, p<0.05 vs LPS+NS; n = 8 mice/group. B-E) Mice were treated with protein transport inhibitor Brefeldin A, challenged with LPS and treated with either NS or AT in vivo. Peritoneal leukocytes were isolated and analyzed by flow cytometry for intracellular IL-10 content. Macrophages were gated based on CD11b and F4/80 expression. * p<0.05 vs LPS+NS; n = 4 mice/group.