Fig. 7. In vivo CREB/CREM binding activity into the Slc2a4 CRE increases in extensor digitorum longus (EDL) of spontaneously hypertensive rats. CREB/CREM binding activity into de Slc2a4-CRE binding site was evaluated by chromatin immunoprecipitation (ChIP) assay in EDL (A) and soleus (B) muscles from Wistar (W) and spontaneously hypertensive (S) rats. Muscle DNA was extracted, fragmented, immunoprecipitated with anti-CREB/CREM antibody or not, and the -670/-480 sequence of Slc2a4 gene was amplified by qPCR. Results of antibody-treated samples were subtracted for calculation of the final relative binding activity. Data are mean ▒ SEM of 3 animals. *P<0.05, unpaired two-tailed Student`s t-test.