Fig. 2. Ceramide does not increase in bronchial epithelial cells upon inhalation of sphingosine. Paraffin sections from biopsies of the pigs were obtained as above and stained with Cy3-labelled anti-ceramide antibodies. Displayed are representative immune stainings from 4 pigs (A) and the quantitative analysis of the fluorescence intensity of a total of 1200 cells per group (B). Given is the mean ± SD, *p<0.05, **p<0.01, ***p<0.001, ANOVA.