**Fig. 6.** (A) and (B) Histogram
showing cell cycle analysis of GC1 cells over expressing Aire and control
Vector (C). Percentage of cells in Sub G_{0}, G_{1}, S and G_{2}/M
stages of cell cycle. Data represented here is from four independent
transfection represented as Mean ± SEM. p<0.05 for G_{0} and
p<0.05 for G_{1} phase. Asterisks indicate statistically significant
difference versus control. (D) Flow cytometric analysis of Annexin positive
cells in GC1 cells, transfected with Vector, CARD and Aire full length are
shown. The Q4 population represents the apoptotic annexin positive cells. (E)
Graphical representation of percentage of annexin positive cells normalised to
vector controls from three independent experiments. Values are represented as
Mean ± SEM and Students t-test showed p< 0.001 for card and p< 0.05 for
full length.